Molecular Diagnosis of Cancer: Methods and Protocols 2nd by Joseph E. Roulston, John M. S. Bartlett

By Joseph E. Roulston, John M. S. Bartlett

A various selection of conveniently reproducible equipment to be used in melanoma detection. Highlights contain FISH-based methodologies presently utilized in the prognosis of strong tumors, the molecular prognosis of genetic abnormalities via DNA array technologies-including sequence-specific oligonucleotide arrays and CGH arrays-and methodologies directed on the detection of epigenetic occasions and at quantitative gene expression. The authors observe those novel diagnostic systems to a large diversity of situations taken from cytology, reliable tumor pathology, hematology, and infrequent mobile detection, paying particular awareness to strength destiny advancements and the sensible difficulties of facing qc and accuracy.

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Ward B. , Cruickshank, D. , Tucker D. , et al. (1987) Independent expression in serum of three tumor-associated antigens: CA125, placental alkaline phosphatase and HMFG2 in human ovarian carcinoma. Br. J. Obstet. Gynæcol. 94, 696–698. 13. Bormer, O. , and Nustad K. (1998) Sensible use of tumor markers in routine practice. Proc. UK NEQAS Meeting 3, 140–145. 14. Hayes, D. , Bast, R. , Desch, C. , et al. (1996) Tumor marker utility grading system: a framework to evaluate clinical utility of tumor markers.

DAKO (Ely, Cambridgeshire, UK), Novocastra Laboratories Ltd. (Newcastle upon Tyne, UK). , Fullerton, CA); clone 3B5 Oncogene Research Products (CN Biosciences, UK), clones CBE-1 and TAB 250, Zymed Laboratories Inc. (San Francisco, CA). Source: Reprinted from ref. 53 with permission from the American Journal of Clinical Pathology. Fig. 1. A diagram of a cell line standard for HER-2/neu, showing the names of the four-cell lines, the position of the cell lines in the paraffin section mounted on a glass slide, and the expected and most appropriate results (in parentheses) following IHC assay and evaluation with the CTA scoring system.

R. (1975) Beyond Normality: The Predictive Value and Efficiency of Medical Diagnoses, Wiley Medical, New York. 8. Bayes, T. (1763) An essay toward solving a problem in the doctrine of chance. Phil. Trans. R. Soc. 53, 370–418. 9. Miller, A. B. (1985) Principles of screening and of the evaluation of screening programs, in Screening for Cancer, (Miller, A. ), Academic, New York, pp. 3–24. 10. Roulston, J. E. and Leonard, R. C. F. (1993) Serological Tumor Markers: An Introduction, Churchill Livingstone, Edinburgh, pp.

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