By Derek LeRoith MD PhD, Jerrold M. Olefsky MD, Simeon I. Taylor MD PhD
Nationwide Institutes of future health, Bethesda, MD. entire reference encompasses the most important advances within the simple molecular, mobile, and genetic elements of diabetes. additionally discusses the traditional and new healing modalities. issues comprise insulin secretion and motion, being pregnant, medical issues, and extra. prior version: c2000. DNLM: Diabetes Mellitus.
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Extra info for Diabetes Mellitus: A Fundamental and Clinical Text
Naylor B, Toivonem T. The use of toluidine blue-stained wet films in diagnostic cytology. Cytopathol Ann . 1993;279â€“287. Gill G. Personal communication. Coughlin D, Lukeman JM. The use of saponin for hemolysis in effusion cytology. Acta Cytol . 1982;26:739. Boon ME, Drijver JS. RoutineCytologic Staining Techniques: Theoretical Background Practice . London: MacMillan Education; 1986. Baliga M. Personal communication. Abendroth CS, Dabbs DJ. Immunocytochemical staining of unstained versus previously stained cytologic preparations.
Several spray fixatives are commercially available. The smears must be immediately flooded with the spray fixative. The spray can should be held at least 12 inches from the slide. The slightest delay in fixing will result in air-drying of the cells, which will stain poorly with the Papanicolaou method. Once sprayed, the fixative on the slide must be completely dried (do not enclose the wet slides in a cardboard folder, as cellular material will adhere to the cardboard folder and be lost forever).
Thus all threeâ€”the neutral salt, cation, and anionâ€”can color different components of the cell. Because of this threefold action, a neutral stain can produce a very useful differentiation of cell components in one staining solution. The two components of the stain include methylene blue (basic dye) and eosin Y (acid dye). The combination of these two dyes result in staining the nucleus purple, and the nucleolus and cytoplasm blue. Any stain that contains a combination of these two dyes is called Romanowsky-type stain, and the results are referred to as Romanowsky effect.